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How To Extract Dna At Home - Science/Technology - YukHub

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How To Extract Dna At Home by Aloyzeus(m): 02:52pm On 7 Jul
#DrenFiles_Experiment
By: Dren

Intro:
In this simple home science project, kids can learn how to extract DNA from their cheek cells using mostly common household items.


Deoxyribonucleic acid (DNA) is a chemical found in the nucleus of cells that contains the blueprint for the development and function of living organisms. It's compared to a set of blueprints since it contains the instructions on how to build cells. The instructions are divided into segments along a strand of DNA and are called genes. Genes provide the code for the production of a protein and control hereditary characteristics such as eye color or personality behaviors. Proteins determine cell type and function, so a cell knows whether it is a skin cell, a blood cell, a bone cell, etc, and how to perform its duties.


What You Need:
Beaker
Test tube with tight fitting lid
Lab scoop
Isopropyl or ethyl alcohol (at least
70% concentration, higher is better—we used 95%), chilled in freezer for several hours
Pipette (if alcohol doesn't come in a
dropper bottle)
Paper cup
Safety goggles
Lab apron
Gloves
Sodium chloride (salt)
Distilled or bottled water
Liquid dish soap or hand soap
Stir rod or wooden skewer (optional)
Small glass vial (optional)



What You Do:

1. Create a saline solution in a beaker by adding two lab scoops of salt to approximately 25 ml of distilled water. Stir until the salt is completely dissolved.


2. Pour the saltwater into the paper cup.


3. Without swallowing, drink a mouthful of the solution from the paper cup and swish it back and forth for at least 30 seconds,occasionally scraping your teeth along the inside of your cheeks as you do. It's best to do this with a clean mouth, i.e. not right after lunch.


4. Spit your mouthwash solution back into the cup. Then bend the cup into a sort of spout and pour the mouthwashed solution into the test tube until it fills about one-half inch of the bottom of the test tube.


5. Carefully add two drops of the liquid soap.


6. Tilting the test tube approximately 45 degrees, use the pipette (or dropper on the alcohol bottle) to add 20 drops of the chilled alcohol so it slides down the test tube without disturbing the solution. Since it's less dense, the alcohol will sit atop the mouthwash and soap solution.


7. Tightly put the cap on the test tube and very slowly and gently tilt it upside down then right side up three times. Do it carefully so as not to make bubbles.


8. Let the test tube sit undisturbed in an upright position for one minute. At this point, you should begin to see a milky white thread, possibly interspersed with bubbles,appear between the solution and the alcohol. That's your DNA! After several minutes, the DNA should be suspended in the alcohol layer.


9. If you wish, insert your skewer or stir rod into the test tube and gently wind the DNA around it.


10. To save it, carefully scrape it into the small vial with a few drops of alcohol.Stored in the freezer, you can preserve your DNA almost indefinitely!


~Dren

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Re: How To Extract Dna At Home by johneewalker: 02:56pm On 7 Jul
Is it a tested and trusted method?
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Re: How To Extract Dna At Home by Aloyzeus(m): 03:09pm On 7 Jul
Yes it tested,ive conducted this for my Project a year ago.
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Re: How To Extract Dna At Home by Kingvictor(m): 05:17pm On 7 Jul
Very Informative
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Re: How To Extract Dna At Home by Aloyzeus(m): 05:19pm On 7 Jul
Thnx.Well Im thinkng also of sharing here my articles i made.
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Re: How To Extract Dna At Home by Kingvictor(m): 05:44pm On 7 Jul
Aloyzeus:Thnx.Well Im thinkng also of sharing here my articles i made.
That would be really nice.. I would be Looking forward to more of your articles
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Re: How To Extract Dna At Home by Aloyzeus(m): 01:04pm On 7 Jul
Yes ill be posting soon ^^ Ill just translate them coz they are in local language.
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Re: How To Extract Dna At Home by johneewalker: 01:25pm On 7 Jul
greetings. pls, after posting, you can also share the topic on social media, esp twitter and googleplus. thanks
have a nice weekend..

Aloyzeus:Yes ill be posting soon ^^ Ill just translate them coz they are in local language.

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